Human Sphingosine-1-phosphate phosphatase 1 (SGPP1) ELISA Kit,Abbkine,KTE60671
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Platform ID
BAB887451963
Abbkine
Contact
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Specifications
Scientific Background
Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides . The deduced 430-amino acid mouse protein shares 17% amino acid identity with yeast Lbp1. The deduced 441-amino acid protein shares 76% amino acid identity with its mouse homolog. Transfection of HEK293 or Chinese hamster ovary (CHO) cells with SGPP1 resulted in marked increase in SGPP1 activity in membrane fractions. SGPP1 was highly specific toward long-chain sphingoid base phosphates and degraded S1P, dihydro-S1P, and phyto-S1P. SGPP1 activity was independent of any cation requirements, including Mg(2+), and was not inhibited by EDTA but was inhibited by Zn(2+).
Synonyms
SGPP1; SPPase1; sphingosine-1-phosphatase
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