Human WAP four-disulfide core domain protein 5 (WFDC5) ELISA Kit,Abbkine,KTE60035
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Platform ID
BAB201057682
Abbkine
Contact
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Specifications
Scientific Background
WFDC5 encodes a member of the WAP-type four-disulfide core (WFDC) domain family. Most WFDC proteins contain only one WFDC domain, and this encoded protein contains two WFDC domains. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. The deduced 123-amino acid protein contains an N-terminal signal peptide, followed by 2 WAP domains. Each WAP domain contains 8 cysteines that form conserved disulfide bridges, and each WAP domain forms a proposed protease-binding loop and a beta sheet. The mature 99-amino acid WAP1 protein has a calculated molecular mass of 10.9 kD.
Synonyms
WFDC5; PRG5; WAP1; dJ211D12.5; p53-responsive gene 5; protease inhibitor WAP1
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