Hypoxia Inducible Factor 1 Alpha (HIF1A) Antibody,Abbexa,abx239939
Hypoxia Inducible Factor 1 Alpha (HIF1A) Antibody is a Mouse Monoclonal antibody for the detection of HIF1a. Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256,.PMID: 7539918).
Host
Mouse
Reactivity
Human
Application
ELISA, WB, IF/ICC
Conjugate
Unconjugated
Platform ID
BAB992050928
Abbexa
Contact
Tel: +44 (0) 1223 755950
Fax: +44 (0) 1223 755951
Email:
Product Specifications
Specifications
NameHypoxia Inducible Factor 1 Alpha (HIF1A) Antibody
Cat. No.abx239939
Accession NumberQ16665
Gene ID (Entrez)3091
HostMouse
IsotypeIgG1
ReactivityHuman
ConjugationUnconjugated
ApplicationELISA, WB, IF/ICC
Working DilutionsWB: 1/1000 - 1/10000, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
ClonalityMonoclonal
Concentration2 mg/ml
Molecular WeightObserved MW: 120 kDa
TargetHIF1A
Immunogenhypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
PurityPurified by Protein A and Protein G affinity chromatography.
Appearance/FormLiquid
FormulationPBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
StorageAliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Regulatory StatusResearch Use Only
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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