Iba1/AIF-1 (E4O4W) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®532 Conjugate)#77748,Cell Signaling Technology (CST),77748

Iba1/AIF-1 (E4O4W) XP®Rabbit mAb (SignalFlexTM Alexa Fluor®532 Conjugate) recognizes endogenous levels of total Iba1/AIF-1 protein.

Host

Rabbit

Reactivity

Human, Mouse, Rat, Hamster, Monkey

Conjugate

SignalFlexTMAlexa Fluor®532 Conjugate

Platform ID

BAB697422072

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameIba1/AIF-1 (E4O4W) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®532 Conjugate)#77748
Cat. No.77748
Accession NumberP55008
Gene ID (Entrez)55008
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat, Hamster, Monkey
ConjugationSignalFlexTMAlexa Fluor®532 Conjugate
ImmunogenIgG
FormulationSignalFlexTM conjugates are produced using highly validated Cell Signaling Technology®primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammatory factor 1 (AIF-1), is an evolutionarily conserved cytoplasmic calcium binding protein containing a central pair of EF-hand calcium binding motifs (1,2). Iba1/AIF-1 was originally cloned from activated macrophages in human atherosclerotic allogenic heart grafts undergoing chronic transplant rejection as well as from rat monocytes (3,4). Its function is not very well understood, but Iba1/AIF-1 expression is upregulated in response to interferon-gamma and, therefore, could modulate macrophage-dependent immune response (3). As an F-actin-binding protein, Iba1/AIF-1 may function to remodel the actin cytoskeleton and contribute to morphological changes that correlate with various microglial/macrophage states (5). Iba1/AIF-1 is also uniquely expressed in cells of monocytic lineage and is, therefore, widely used as a marker for microglia/macrophages in the brain and other tissue (6).Schulze, J.O. et al. (2008)FEBS J275, 4627-40.Deininger, M.H. et al. (2002)FEBS Lett514, 115-21.Utans, U. et al. (1995)J Clin Invest95, 2954-62.Imai, Y. et al. (1996)Biochem Biophys Res Commun224, 855-62.Kanazawa, H. et al. (2002)J Biol Chem277, 20026-32.Ito, D. et al. (1998)Brain Res Mol Brain Res57, 1-9.Alternate NamesAIF-1; AIF1; Allograft inflammatory factor 1; G1; IBA 1; IBA-1; IBA1; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; IRT-1; IRT1; Protein G1

Synonyms

AIF-1; AIF1; Allograft inflammatory factor 1; G1; IBA 1; IBA-1; IBA1; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; IRT-1; IRT1; Protein G1

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