Immunotag^TMDYKDDDDK-tag Polyclonal Antibody,G-Biosciences,ITG0004

The DYKDDDDK peptide has been used extensively as a general tag in expression vectors. Often referred to in literature as the FLAG tag (FLAG is a registered trademark of MERCK), this peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DYKDDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DYKDDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DYKDDDDK peptide is more likely to be accessible to antibodies. A DYKDDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag