ImmunotagTMHis-tag Polyclonal Antibody,G-Biosciences,ITG0002

Host

Rabbit

Reactivity

All

Application

WB,ELISA

Platform ID

BAB533139125

G-Biosciences

Headquarters

9800 Page Avenue St. Louis, MO 63132-1429 U.S.A.

Contact

Tel: 800-628-7730
Fax: 314-991-1504

Product Specifications
Scientific Background
Synonyms

Specifications

NameImmunotagTMHis-tag Polyclonal Antibody
Cat. No.ITG0002
HostRabbit
ReactivityAll
ApplicationWB,ELISA
Working DilutionsWB: 1:1000-1:3000, EIA: 1:20000
ClonalityPolyclonal
Concentration1 mg/ml
TargetHis-Tag
Immunogen6X His synthetic peptide conjugated to KLH.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epyope-specific immunogen.
Appearance/FormLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.0.5% sodium azide.
Storage-20°C/1 year

Scientific Background

The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

Synonyms

6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.