LC3B (E5Q2K) Mouse Monoclonal Antibody (SignalFlex^TMPacific Blue^TMConjugate)#17720,Cell Signaling Technology (CST),17720

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).Reggiori, F. and Klionsky, D.J. (2002)Eukaryot. Cell1, 11-21.Codogno, P. and Meijer, A.J. (2005)Cell Death Differ.12 Suppl 2, 1509-18.Levine, B. and Yuan, J. (2005)J. Clin. Invest.115, 2679-88.Mann, S.S. and Hammarback, J.A. (1994)J. Biol. Chem.269, 11492-97.Lang, T. et al. (1998)EMBO J.17, 3597-607.Kabeya, Y. et al. (2000)EMBO J.19, 5720-28.He, H. et al. (2003)J. Biol. Chem.278, 29278-87.Tanida, I. et al. (2004)J. Biol. Chem.279, 47704-10.Wu, J. et al. (2006)Biochem. Biophys. Res. Commun.339, 437-42.Ichimura, Y. et al. (2000)Nature408, 488-92.Kabeya, Y. et al. (2004)J. Cell Sci.117, 2805-12.Alternate NamesATG8F; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1 light chain 3-like protein 2; MAP1A/1BLC3; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1ALC3; MAP1LC3B; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3B; MLP3B

ATG8F; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1 light chain 3-like protein 2; MAP1A/1BLC3; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1ALC3; MAP1LC3B; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3B; MLP3B