LDHA (F3T9U) Rabbit Monoclonal Antibody#71368,Cell Signaling Technology (CST),71368

LDHA (F3T9U) Rabbit Monoclonal Antibody recognizes endogenous levels of total LDHA protein. This antibody does not cross-react with LDHB or LDHC protein.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Application

Western Blotting: 1:1000 Simple WesternTM: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Frozen): 1:400 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600

Platform ID

BAB047710175

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameLDHA (F3T9U) Rabbit Monoclonal Antibody#71368
Cat. No.71368
Accession NumberP06151
Gene ID (Entrez)06151, 16828
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat
ApplicationWestern Blotting: 1:1000 Simple WesternTM: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Frozen): 1:400 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600
Molecular Weight37
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.Semenza, G.L. et al. (1996)J Biol Chem271, 32529-37.Semenza, G.L. (2007)Biochem J405, 1-9.Alternate NamesL-lactate dehydrogenase A chain; l7R2; lactate dehydrogenase 1, A chain; lactate dehydrogenase A; lactate dehydrogenase A4; lactate dehydrogenase-A; LDH; LDH muscle subunit; Ldh-; Ldh-1; LDH-A; LDH-M; Ldh1; Ldha; Ldhm; M-LDH; OTTMUSP00000017774

Synonyms

L-lactate dehydrogenase A chain; l7R2; lactate dehydrogenase 1, A chain; lactate dehydrogenase A; lactate dehydrogenase A4; lactate dehydrogenase-A; LDH; LDH muscle subunit; Ldh-; Ldh-1; LDH-A; LDH-M; Ldh1; Ldha; Ldhm; M-LDH; OTTMUSP00000017774

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