MIF (E8S8H) Rabbit Monoclonal Antibody#75038,Cell Signaling Technology (CST),75038

MIF (E8S8H) Rabbit Monoclonal Antibody recognizes endogenous levels of total MIF protein.

Host

Rabbit

Reactivity

Human, Mouse, Rat, Hamster

Application

Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:100 - 1:200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200

Platform ID

BAB050009071

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameMIF (E8S8H) Rabbit Monoclonal Antibody#75038
Cat. No.75038
Accession NumberP14174
Gene ID (Entrez)14174, 4282
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat, Hamster
ApplicationWestern Blotting: 1:1000 Immunofluorescence (Frozen): 1:100 - 1:200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200
Molecular Weight12
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

MIF (macrophage migration inhibitory factor) is a pleiotropic cytokine that stimulates pro-inflammatory, chemotactic, and growth responses in cells (1). MIF binds its cognate receptor (a CD74/CD44 complex) to activate multiple signaling pathways such as Src, ERK, MAPK, Akt, and suppress p53-induced apoptosis (2). The interaction of MIF with non-cognate chemokine receptors CXCR2 and CXCR4 promotes chemotaxis that enables recruitment of monocytes/neutrophils and T cells (3). During an innate immune response, MIF has been shown to repress the inhibitory effects of glucocorticoids on macrophages and T cells, thus promoting host inflammation and immunity (4). MIF may also play roles in the progression of other disease processes, including cancer cell proliferation and metastasis, and angiogenesis (5), atherosclerotic plaque formation following myocardial ischemia (6), and autoimmune pathogenesis (7). MIF has thus been proposed as a promising therapeutic drug target for multiple indications.Sparkes, A. et al. (2017)Immunobiology222, 473-82.Flaster, H. et al. (2007)Mol Endocrinol21, 1267-80.Tillmann, S. et al. (2013)Front Immunol4, 115.Calandra, T. and Bucala, R. (2017)Crit Rev Immunol37, 359-70.Kindt, N. et al. (2016)Oncol Lett12, 2247-53.Tilstam, P.V. et al. (2017)Expert Opin Ther Targets21, 671-83.Bilsborrow, J.B. et al. (2019)Expert Opin Ther Targets23, 733-44.Alternate Namesepididymis secretory sperm binding protein; GIF; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase

Synonyms

epididymis secretory sperm binding protein; GIF; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase

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