MRCK alpha (E2S5I) Rabbit Monoclonal Antibody#81681,Cell Signaling Technology (CST),81681

MRCK alpha (E2S5I) Rabbit Monoclonal Antibody recognizes endogenous levels of total MRCKα protein. Based on the sequence of the peptide antigen, this antibody is not expected to cross-react with MRCKβ or MRCKγ.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:12800

Platform ID

BAB363314934

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameMRCK alpha (E2S5I) Rabbit Monoclonal Antibody#81681
Cat. No.81681
Accession NumberQ5VT25
Gene ID (Entrez)8476
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:12800
Molecular Weight200
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

CDC42BPA/MRCK (Myotonic dystrophy kinase-related CDC42-binding kinase) comprises a family of serine/threonine kinases that function as downstream effectors of the small GTPase, CDC42. Three MRCK family members have been identified: MRCKα, MRCKβ, and MRCKγ. MRCK family kinases have some substrates in common with the Rho-associated kinases, ROCK1 and ROCK2, including Myosin Light Chain 2 (MLC2). Signaling through both ROCK and MRCK kinase families regulates actin dynamics, cell migration, invasion, and metastasis (1,2). Simultaneous inhibition of both ROCK and MRCK kinases prevents migration and invasion of human cancer cell lines (3). Small molecule inhibitors targeting the MRCK family are being investigated as potential treatments for glioblastoma and skin cancer (4,5).Kale, V.P. et al. (2015)Cancer Lett361, 185-96.Zhao, Z. and Manser, E. (2015)Small GTPases6, 81-8.Kale, V.P. et al. (2014)Cancer Lett354, 299-310.Birch, J.L. et al. (2018)Cancer Res78, 6509-6522.Unbekandt, M. et al. (2018)Cancer Res78, 2096-2114.Alternate NamesCDC42 binding protein kinase alpha; CDC42 binding protein kinase alpha (DMPK-like); CDC42 binding protein kinase beta; CDC42 binidng protein kinase beta; CDC42-binding protein kinase alpha; CDC42-binding protein kinase alpha (DMPK-like); CDC42BPA; DKFZp686L1738; DKFZp686P1738; DMPK-like alpha; FLJ23347; KIAA0451; MRCK; MRCK alpha; MRCKA; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; myotonic dystrophy kinase-related CDC42-binding protein kinase alpha; Myotonic dystrophy protein kinase-like alpha; PK428; ser-thr protein kinase PK428; ser-thr protein kinase related to the myotonic dystrophy protein kinase; Serine/threonine-protein kinase MRCK alpha

Synonyms

CDC42 binding protein kinase alpha; CDC42 binding protein kinase alpha (DMPK-like); CDC42 binding protein kinase beta; CDC42 binidng protein kinase beta; CDC42-binding protein kinase alpha; CDC42-binding protein kinase alpha (DMPK-like); CDC42BPA; DKFZp686L1738; DKFZp686P1738; DMPK-like alpha; FLJ23347; KIAA0451; MRCK; MRCK alpha; MRCKA; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; myotonic dystrophy kinase-related CDC42-binding protein kinase alpha; Myotonic dystrophy protein kinase-like alpha; PK428; ser-thr protein kinase PK428; ser-thr protein kinase related to the myotonic dystrophy protein kinase; Serine/threonine-protein kinase MRCK alpha

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