Mcl-1 (D2W9E) Rabbit Monoclonal Antibody (SignalFlex^TMmFluor^TMRed 780 Conjugate)#74857,Cell Signaling Technology (CST),74857

Mcl-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway (1). Similar to other Bcl-2 family members, Mcl-1 localizes to the mitochondria (2), interacts with and antagonizes pro-apoptotic Bcl-2 family members (3), and inhibits apoptosis induced by a number of cytotoxic stimuli (4). Mcl-1 differs from its other family members in its regulation at both the transcriptional and posttranslational level. First, Mcl-1 has an extended amino-terminal PEST region, which is responsible for its relatively short half-life (1,2). Second, unlike other family members, Mcl-1 is rapidly transcribed via a PI3K/Akt dependent pathway, resulting in its increased expression during myeloid differentiation and cytokine stimulation (1,5-7). Mcl-1 is phosphorylated in response to treatment with phorbol ester, microtubule-damaging agents, oxidative stress, and cytokine withdrawal (8-11). Phosphorylation at Thr163, the conserved MAP kinase/ERK site located within the PEST region, slows Mcl-1 protein turnover (10) but may prime the GSK-3 mediated phosphorylation at Ser159 that leads to Mcl-1 destabilization (11). Mcl-1 deficiency in mice results in peri-implantation lethality (12). In addition, conditional disruption of the correspondingmcl-1gene shows that Mcl-1 plays an important role in early lymphoid development and in the maintenance of mature lymphocytes (13).Kozopas, K.M. et al. (1993)Proc Natl Acad Sci USA90, 3516-20.Yang, T. et al. (1995)J Cell Biol128, 1173-84.Sato, T. et al. (1994)Proc Natl Acad Sci USA91, 9238-42.Zhou, P. et al. (1997)Blood89, 630-43.Wang, J.M. et al. (1999)Mol Cell Biol19, 6195-206.Jourdan, M. et al. (2003)Oncogene22, 2950-9.Chao, J.R. et al. (1998)Mol Cell Biol18, 4883-98.Domina, A.M. et al. (2000)J Biol Chem275, 21688-94.Inoshita, S. et al. (2002)J Biol Chem277, 43730-4.Domina, A.M. et al. (2004)Oncogene23, 5301-15.Maurer, U. et al. (2006)Mol Cell21, 749-60.Rinkenberger, J.L. et al. (2000)Genes Dev14, 23-7.Opferman, J.T. et al. (2003)Nature426, 671-6.Alternate NamesAW556805; Bcl-2-related protein EAT/mcl1; Induced myeloid leukemia cell differentiation protein Mcl-1 homolog; Mcl-; Mcl-1; Mcl1; myeloid cell leukemia sequence 1

AW556805; Bcl-2-related protein EAT/mcl1; Induced myeloid leukemia cell differentiation protein Mcl-1 homolog; Mcl-; Mcl-1; Mcl1; myeloid cell leukemia sequence 1