Miwi (D92B7) Rabbit Monoclonal Antibody#6915,Cell Signaling Technology (CST),6915

Miwi (D92B7) Rabbit Monoclonal Antibody recognizes endogenous levels of total Miwi protein. Non-specific staining was observed in a subset of mouse immune cells by immunohistochemistry.

Host

Rabbit

Reactivity

Human, Mouse

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Immunofluorescence (Frozen): 1:800

Platform ID

BAB300134011

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameMiwi (D92B7) Rabbit Monoclonal Antibody#6915
Cat. No.6915
Accession NumberQ9JMB7
Gene ID (Entrez)57749
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Immunofluorescence (Frozen): 1:800
Molecular Weight90
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

TheDrosophilapiwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species includingArabidopsis,C. elegans,andHomo sapiens(1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).Cox, D.N. et al. (1998)Genes Dev12, 3715-27.Kuramochi-Miyagawa, S. et al. (2001)Mech Dev108, 121-33.Aravin, A. et al. (2006)Nature442, 203-7.Grivna, S.T. et al. (2006)Proc Natl Acad Sci USA103, 13415-20.Grivna, S.T. et al. (2006)Genes Dev20, 1709-14.Alternate NamesMGC150073; Miwi; piwi like homolog 1; piwi-like homolog 1; piwi-like homolog 1 (Drosophila); Piwi-like protein 1; piwi-like RNA-mediated gene silencing 1; Piwil1; PIWL1

Synonyms

MGC150073; Miwi; piwi like homolog 1; piwi-like homolog 1; piwi-like homolog 1 (Drosophila); Piwi-like protein 1; piwi-like RNA-mediated gene silencing 1; Piwil1; PIWL1

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