MonoMethyl-Histone H3-K4 Rabbit mAb- ABclonal,ABclonal,A27915

Reactivity

Human, Mouse, Rat, Arabidopsis, Rice, Wheat, Other (Wide Range Predicted)

Application

WB, IF/ICC, IHC-P, DB, ChIP, ELISA

Conjugate

Unconjugated

Platform ID

BAB720133694

ABclonal

Headquarters

500W Cummings Park, Ste. 6500 Woburn, MA 01801

ABclonal

Contact

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Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameMonoMethyl-Histone H3-K4 Rabbit mAb- ABclonal
Cat. No.A27915
Accession NumberQ16695
Gene ID (Entrez)8290
IsotypeIgG
ReactivityHuman, Mouse, Rat, Arabidopsis, Rice, Wheat, Other (Wide Range Predicted)
ConjugationUnconjugated
ApplicationWB, IF/ICC, IHC-P, DB, ChIP, ELISA
Working DilutionsWB:1:4000 - 1:20000 | IF/ICC:1:200 - 1:1000 | IHC-P:1:5000 - 1:20000 | DB:1:1000 - 1:2000 | ChIP:0.5μg antibody for 10μg-15μg of Chromatin | ELISA:Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (≥1:10000)，a sequential dilution method is strongly recommended to ensure measurement accuracy.
Clone NumberARC69516
Molecular Weight17 kDa
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
PurityAffinity purification
Appearance/FormLiquid
StorageStore at -20℃. Avoid freeze / thaw cycles.; Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Regulatory StatusResearch Use Only

Scientific Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Synonyms

H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Histone H3; H3-4; H3/t; H3/g; H3FT; HIST3H3

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