Mouse Ghrelin O-acyltransferase (MBOAT4) ELISA Kit,Abbkine,KTE71104
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Platform ID
BAB511964697
Abbkine
Contact
Tel: +1-404-854-0155
Fax:
Email:
Specifications
Scientific Background
GOAT could modify ghrelin ser3 with fatty acids up to tetradecanoic acid. Replacement of his338 of GOAT with ala completely abolished the ability of GOAT to octanoylate ghrelin.mouse Goat octanoylated ghrelin (GHRL), a 28-amino acid appetite-stimulating peptide hormone, following cotransfection of Goat and preproghrelin in cultured endocrine cell lines. Mutation analysis showed that Goat octanoylated ghrelin on ser3, a modification required for its endocrine effects. Asp307 and his338 of Goat were required for octanoylation.The mouse and human proteins both contain 435 amino acids and have 8 putative transmembrane segments and conserved catalytic asparagine and histidine residues. Semiquantitative PCR of mouse tissues detected highest Goat expression in stomach, with lower expression in small intestine, colon, and testis.
Synonyms
MBOAT4; FKSG89; GOAT; OACT4; O-acyltransferase (membrane bound) domain containing 4; ghrelin O-acyltransferase
Category Paths
- Products>ELISA & Assay Kits>ELISA Kits
Please provide the required information below so that we can quickly source your products.