Mouse IgG2a [Clone C1.18.4-mIgG2a] — Isotype Control – Purified No Carrier Protein,Leinco,I-1326

The Mouse IgG2a Isotype Control (Clone C1.18.4) is a critical negative control used to measure and eliminate non-specific background binding in assays such as flow cytometry, immunohistochemistry, and ELISA. Traditionally produced via hybridoma, this classic clone has now been engineered as a recombinant antibody expressed in mammalian HEK293 cells. The Recombinant Advantage: While traditional hybridomas are susceptible to genetic drift, loss of antibody expression, or the production of extra endogenous light chains, our recombinant HEK293-expressed C1.18.4 guarantees absolute sequence fidelity. By cloning the exact heavy and light chain sequences from the original hybridoma into a stable HEK293 expression system, this version eliminates the risk of multi-chain impurities. The result is a highly pure, structurally defined isotype control that offers unparalleled lot-to-lot consistency, ensuring that your background measurements remain perfectly reproducible across long-term studies. Furthermore, this version is produced in a serum-free (FBS-free) culture, completely eliminating contaminating bovine IgGs and animal-derived variables to deliver an exceptionally clean background control.