Mouse IgG2b Isotype Control (Clone MPC-11-D265A) – Recombinant In vivo Purified No Carrier Protein,Leinco,I-1325

The Clone MPC-11 recombinant antibody serves as a highly dependable mouse IgG2b negative control for a wide array of immunological assays. Derived from a mouse plasmacytoma, the variable regions of the MPC-11 clone exhibit no specific binding affinity for mammalian target proteins. Because it lacks functional reactivity to human, mouse, or rat tissues, it is the ideal tool for establishing baseline measurements and distinguishing true antigen-specific signals from background noise in flow cytometry, multiplex assays, and functional studies. Targeted D265A Fc Engineering To prevent unwanted interactions with cellular receptors, this specific variant of Clone MPC-11 has been engineered with a D265A point mutation (aspartic acid to alanine substitution at position 265) within the IgG2b Fc domain. Effector Function Silencing: This targeted modification dramatically reduces binding affinity to Fcγ receptors (FcγRI, FcγRII, and FcγRIII) and inhibits C1q complement binding. Assay Clarity: By effectively mitigating Fc-mediated effector functions and non-specific background binding, the D265A mutation ensures a completely inert profile, making it safe and reliable for sensitive in vitro cultures and in vivo animal models.