Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) ELISA Kit,Abbkine,KTE70228
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Platform ID
BAB205655474
Abbkine
Contact
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Specifications
Scientific Background
TIM44, a peripheral inner membrane protein, tethers mitochondrial HSP70 to the import channel. A model peptide substrate rapidly released mitochondrial HSP70 from TIM44, even in the absence of ATP hydrolysis. In vivo, the analogous interaction of translocating polypeptide would release mitochondrial HSP70 from the channel. TIMM44 is hydrophilic and contains no hydrophobic membrane-spanning segments. The N terminus has a coiled-coil motif and a matrix-targeting segment with 6 positively and no negatively charged residues. Western blot analysis showed that TIMM44 is present in membranes as well as matrix and is most likely a 45-kD peripheral membrane protein loosely associated with the inner membrane of mitochondria.
Synonyms
TIMM44; DKFZp686H05241; TIM44; mitochondrial inner membrane translocase; translocase of inner mitochondrial membrane 44
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