Mouse TRAF2 and NCK-interacting protein kinase (TNIK) ELISA Kit,Abbkine,KTE70111

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Platform ID

BAB708304202

Abbkine

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3052 Stroop Hill Road, Apt 203, Atlanta 30303, Georgia, United States of America

Abbkine

Contact

Tel: +1-404-854-0155
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Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameMouse TRAF2 and NCK-interacting protein kinase (TNIK) ELISA Kit
Cat. No.KTE70111
Accession NumberP83510
Gene ID (Entrez)665113
ShippingThe unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
StorageThe unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Regulatory StatusResearch Use Only

Scientific Background

TNIK was autophosphorylated in a manner dependent upon lys54 in the ATP-binding pocket of its kinase domain. Immunoprecipitation analysis showed that epitope-tagged TNIK coprecipitated endogenous TRAF2 from human embryonic kidney cells. Mutation analysis revealed that the intermediate domain was sufficient for the interaction, although the GCK domain may contribute. The intermediate domain was also sufficient for interaction with NCK. Cotransfection of TNIK with JNK2 (MAPK9) enhanced JNK2 kinase activity in a dose-dependent manner, and this effect was mediated by the GCK region of TNIK, but not the kinase domain. TNIK overexpression had no effect on ERK1 (MAPK3), p38 (MAPK14), or NF-kappa-B.

Synonyms

TNIK

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