Mouse Testican-3 (SPOCK3) ELISA Kit,Abbkine,KTE70237
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Platform ID
BAB769885893
Abbkine
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Specifications
Scientific Background
The cDNA was a splice variant of SPOCK3, which the authors called testican-3, and included the N-terminal 313-amino acid region of SPOCK3 with a 3-amino acid substitution at the C terminus; the authors designated the splice variant N-TES. N-TES contains a signal peptide, a unique domain, a follistatin-like domain, and a Ca(2+)-binding domain, but lacks a C-terminal thyroglobulin domain and 2 putative glycosaminoglycan attachment sites of SPOCK3. The full-length SPOCK3 protein contains 436 amino acids and shares 51% and 44% homology with SPOCK1 and SPOCK2 , respectively. Semiquantitative RT-PCR detected expression of SPOCK3 and N-TES transcripts in normal brain; transcripts of both were downregulated in glioma tissues.
Synonyms
SPOCK3; HSAJ1454; TES-3; testican 3
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