NEDD8 (19E3) Rabbit Monoclonal Antibody (BSA and Azide Free)#42039,Cell Signaling Technology (CST),42039

Neural precursor cell-expressed developmentally downregulated protein 8 (NEDD8), also known as Rub1 (related to ubiquitin 1) in plants and yeast, is a member of the ubiquitin-like protein family (1,2). The covalent attachment of NEDD8 to target proteins, termed neddylation, is a reversible, multi-step process analogous to ubiquitination. NEDD8 is first synthesized in a precursor form with a carboxy-terminal extension peptide that is removed by either the UCH-L3 or NEDP1/DEN1 hydrolase protein to yield a mature NEDD8 protein (3,4). Mature NEDD8 is then covalently linked to target proteins via the carboxy-terminal glycine residue in a reaction catalyzed by the APP-BP1/Uba3 heterodimer complex and Ubc12 as the E1- and E2-like enzymes, respectively (5). An E3 ligase protein, Roc1/Rbx1, is also required for neddylation of the cullin proteins (6). Protein de-neddylation is catalyzed by a number of enzymes in the cell, including a "ubiquitin-specific" protease USP21, the NEDP1/DEN1 hydrolase and the COP9/signalosome (CSN) (7,8,9). In contrast to the ubiquitin pathway, the NEDD8 modification system acts on only a few substrates and does not appear to target proteins for degradation. Neddylation of cullin proteins activates the SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complex by promoting complex formation and enhancing the recruitment of the E2-ubiquitin intermediate (10). While NEDD8 modification of VHL is not required for ubiquitination of HIF1-α, it is required for fibronectin matrix assembly (11). Mdm2-dependent neddylation of p53 inhibits its transcriptional activity (12).Chiba, T. and Tanaka, K. (2004)Curr. Protein Pept. Sci.5, 177-184.Schwartz, D.C. and Hochstrasser, M. (2003)Trends Biochem. Sci.28, 321-328.Wada, H. et al. (1998)Biochem. Biophys. Res. Commun.251, 688-692.Hemelaar, J. et al. (2004)Mol. Cell Biol.24, 84-95.Osaka, F. et al. (1998)Genes Dev.12, 2263-2268.Kamura, T. et al. (1999)Genes Dev.13, 2928-2933.Gong, L. et al. (2000)J. Biol. Chem.275, 14212-14216.Mendoza, H.M. et al. (2003)J. Biol. Chem.278, 25637-25643.Lyapina, S. et al. (2001)Science292, 1382-1385.Kawakami, T. et al. (2001)EMBO J.20, 4003-4012.Stickle, N.H. et al. (2004)Mol. Cell Biol.24, 3251-3261.Xirodimas, D.P. et al. (2004)Cell118, 83-97.Alternate NamesFLJ43224; MGC104393; MGC125896; MGC125897; NEDD-8; NEDD8; NEDD8 ubiquitin like modifier; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Ubiquitin-like protein Nedd8

FLJ43224; MGC104393; MGC125896; MGC125897; NEDD-8; NEDD8; NEDD8 ubiquitin like modifier; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Ubiquitin-like protein Nedd8