NK1.1/CD161 (E6Y9G) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®700 Conjugate)#47062,Cell Signaling Technology (CST),47062

NK1.1/CD161 (E6Y9G) Rabbit mAb (SignalFlexTM Alexa Fluor®700 Conjugate) recognizes endogenous levels of total CD161c protein from C57BL/6, SJL, and NZW mice, but not from BALB/c and 129S6 mice. Mice from other strains have not been tested. This antibody is predicted to cross-react with CD161b and may cross-react with CD161a protein in some mouse strains.

Host

Rabbit

Reactivity

Mouse

Conjugate

SignalFlexTMAlexa Fluor®700 Conjugate

Platform ID

BAB388014337

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameNK1.1/CD161 (E6Y9G) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®700 Conjugate)#47062
Cat. No.47062
Accession NumberP27814
Gene ID (Entrez)27814, 17059
HostRabbit
SensitivityEndogenous
ReactivityMouse
ConjugationSignalFlexTMAlexa Fluor®700 Conjugate
ImmunogenIgG
FormulationSignalFlexTM conjugates are produced using highly validated Cell Signaling Technology®primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD161/KLRB1 (Killer cell lectin-like receptor subfamily B member 1, also known as CLEC5B and NKR-P1A) is a type II transmembrane protein that is expressed on the majority of Natural Killer (NK) cells, NK T cells, and some T lymphocytes (1). CD161/KLRB1 is also expressed on Th17 cells, promotes their generation, and modulates their function (2). Engagement with its ligand lectin-like transcript 1 (LLT1) inhibits NK cell function, while LLT1 and CD161/KLRB1 interaction in the presence of a TCR signal enhances IFN-gamma production by T cells (3,4). There are several different CD161 isoforms in rodents and some function as activating receptors as well (5,6).There is a family of Klrb1 genes in rodents (7). PK136 antibody recognizes a specific epitope on mouse KLRB1b/c, also referred to as NK1.1, and is commonly used for detection of mouse NK cells in certain mouse strains (CE, B6, NZB, C58, Ma/My, ST, SJL, FVB). However, the epitope is absent in other mouse strains (BALB/c, AKR, CBA, C3H, DBA, 129) (8).Lanier, L.L. et al. (1994)J Immunol153, 2417-28.Bai, A. et al. (2014)J Immunol193, 3366-77.Aldemir, H. et al. (2005)J Immunol175, 7791-5.Rosen, D.B. et al. (2005)J Immunol175, 7796-9.Carlyle, J.R. et al. (2006)J Immunol176, 7511-24.Kirkham, C.L. and Carlyle, J.R. (2014)Front Immunol5, 214.Kirkham, C.L. and Carlyle, J.R. (2014)Front Immunol5, 214.Carlyle, J.R. et al. (2006)J Immunol176, 7511-24.Alternate NamesAI462337; CD161; CD161 antigen-like family member C; CD161c; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1C; Killer cell lectin-like receptor subfamily B member 1C; Klrb1b; Klrb1c; KLRBC; Ly-5; Ly-55c; Ly-59; Ly5; Ly55; Ly55c; Ly59; lymphocyte antigen 55 complex, locus C; Lymphocyte antigen 55c; lymphocyte antigen 59; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; NK-; Nk-1; NK-1.; Nk-1.2; NK-RP1; Nk1; Nk1.; NK1.1; Nk1.2; NKR; NKR-; NKR-P1 40; NKR-P1.9; NKR-P1B; NKR-P1C; Nkrp; NKRP1; NKRP140; Nkrp1b; Nkrp1c

Synonyms

AI462337; CD161; CD161 antigen-like family member C; CD161c; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1C; Killer cell lectin-like receptor subfamily B member 1C; Klrb1b; Klrb1c; KLRBC; Ly-5; Ly-55c; Ly-59; Ly5; Ly55; Ly55c; Ly59; lymphocyte antigen 55 complex, locus C; Lymphocyte antigen 55c; lymphocyte antigen 59; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; NK-; Nk-1; NK-1.; Nk-1.2; NK-RP1; Nk1; Nk1.; NK1.1; Nk1.2; NKR; NKR-; NKR-P1 40; NKR-P1.9; NKR-P1B; NKR-P1C; Nkrp; NKRP1; NKRP140; Nkrp1b; Nkrp1c

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