Neurofilament-M (E7Y8W) Mouse Monoclonal Antibody#53086,Cell Signaling Technology (CST),53086

Neurofilament-M (E7Y8W) Mouse Monoclonal Antibody recognizes endogenous levels of total Neurofilament-M protein.

Host

Mouse

Reactivity

Human, Mouse, Rat

Application

Western Blotting: 1:1000 Simple WesternTM: 1:10 - 1:50 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800

Platform ID

BAB490076588

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameNeurofilament-M (E7Y8W) Mouse Monoclonal Antibody#53086
Cat. No.53086
Accession NumberP07197
Gene ID (Entrez)07197, 4741
HostMouse
SensitivityEndogenous
ReactivityHuman, Mouse, Rat
ApplicationWestern Blotting: 1:1000 Simple WesternTM: 1:10 - 1:50 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Molecular Weight160
ImmunogenIgG2a
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #60044.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #60044.
Regulatory StatusResearch Use Only

Scientific Background

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).Al-Chalabi, A. and Miller, C.C. (2003)Bioessays25, 346-55.Cohlberg, J.A. et al. (1995)J Biol Chem270, 9334-9.Hisanaga, S. et al. (1994)Mol Biol Cell5, 161-72.Sihag, R.K. et al. (1999)J Neurochem72, 491-9.Alternate Names160 kDa neurofilament protein; NEF3; NEFM; Neurofilament 3; Neurofilament M; neurofilament medium; Neurofilament medium polypeptide; Neurofilament triplet M protein; neurofilament-3 (150 kD medium); neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; NF-M; NFM

Synonyms

160 kDa neurofilament protein; NEF3; NEFM; Neurofilament 3; Neurofilament M; neurofilament medium; Neurofilament medium polypeptide; Neurofilament triplet M protein; neurofilament-3 (150 kD medium); neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; NF-M; NFM

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