Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit Monoclonal Antibody (SignalFlexTMmFluorTMUV375 Conjugate)#54611,Cell Signaling Technology (CST),54611

Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (SignalFlexTM mFluorTM UV375 Conjugate) recognizes endogenous β-catenin protein when residues Ser33, Ser37, and Thr41 are not phosphorylated. It does not detect β-catenin protein if tri-phosphorylated at Ser33/Ser37/Thr41. This antibody may also detect β-catenin protein when singly phosphorylated at Ser33. This specificity data was derived from competition ELISA and dot blot analysis using synthetic peptides.

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Conjugate

SignalFlexTMmFluorTMUV375 Conjugate

Platform ID

BAB263151771

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameNon-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit Monoclonal Antibody (SignalFlexTMmFluorTMUV375 Conjugate)#54611
Cat. No.54611
Accession NumberP35222
Gene ID (Entrez)35222, 1499
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat, Monkey
ConjugationSignalFlexTMmFluorTMUV375 Conjugate
ImmunogenIgG
FormulationSignalFlexTM conjugates are produced using highly validated Cell Signaling Technology®primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb is designed to specifically recognize the stabilized form of β-catenin, i.e., protein that has not been phosphorylated by GSK-3, and thus is functionally active in cell-cell adhesion and/or the canonical Wnt signaling pathway.Cadigan, K.M. and Nusse, R. (1997)Genes Dev11, 3286-3305.Wodarz, A. and Nusse, R. (1998)Annu Rev Cell Dev Biol14, 59-88.Polakis, P. (1999)Curr Opin Genet Dev9, 15-21.Amit, S. et al. (2002)Genes Dev16, 1066-76.Liu, C. et al. (2002)Cell108, 837-47.Yanagawa, S. et al. (2002)EMBO J21, 1733-42.Yost, C. et al. (1996)Genes Dev10, 1443-54.Morin, P.J. et al. (1997)Science275, 1787-90.Alternate Namesarmadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta 1; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; EVR7; FLJ25606; FLJ37923; MRD19; NEDSDV

Synonyms

armadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta 1; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; EVR7; FLJ25606; FLJ37923; MRD19; NEDSDV

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