PE/Cyanine5 anti-mouse CD45 Antibody anti-CD45 - 30-F11,BioLegend,103110

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Host

Rat

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB117344321

BioLegend

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NamePE/Cyanine5 anti-mouse CD45 Antibody anti-CD45 - 30-F11
Cat. No.103110
HostRat
RRIDAB_312974 (BioLegend Cat. No. 103109)AB_312975 (BioLegend Cat. No. 103110)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone Number30-F11
Concentration0.2 mg/ml
TargetCD45
ImmunogenMouse thymus or spleen
PurityThe antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

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