PE/Cyanine5 anti-mouse CD62L Antibody anti-CD62L - MEL-14,BioLegend,104410

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4,in vivoandin vitroblocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Host

Rat

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB690059401

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Contact

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Product Specifications
Scientific Background

Specifications

NamePE/Cyanine5 anti-mouse CD62L Antibody anti-CD62L - MEL-14
Cat. No.104410
HostRat
RRIDAB_313097 (BioLegend Cat. No. 104410)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberMEL-14
Concentration0.2 mg/ml
TargetCD62L
ImmunogenC3H/eb mouse B lymphoma 38C-13
PurityThe antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

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