PE/Cyanine5 anti-mouse I-A/I-E Antibody anti-I-A/I-E - M5/114.15.2,BioLegend,107612
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-EkMHC class II alloantigens from mice carrying H-2p,r,q,b,d,uhaplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-AsMHC class II alloantigens.1Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6,in vitroandin vivoblocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).
Host
Rat
Reactivity
Mouse
Application
FC - Quality tested
Platform ID
BAB874004858
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NamePE/Cyanine5 anti-mouse I-A/I-E Antibody anti-I-A/I-E - M5/114.15.2
Cat. No.107612
HostRat
RRIDAB_313326 (BioLegend Cat. No. 107611)AB_313327 (BioLegend Cat. No. 107612)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberM5/114.15.2
Concentration0.2 mg/ml
TargetI-A/I-E
ImmunogenActivated C57BL/6 mouse spleen cells
PurityThe antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,rbearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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