PE/Cyanine7 anti-human IL-10 Antibody anti-IL-10 - JES3-9D7,BioLegend,501420
ELISA Capture1-5or ELISPOT Capture6:The Ultra-LEAFpurified JES3-9D7 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. No. 501502) as the detecting antibody and recombinant human IL-10 (Cat. No. 571009) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Neutralization1-3,9:The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 501427 & 501428). The JES3-9D7 antibody can neutralize the bioactivity of natural or recombinant IL-10.Additional reported applications (for the relevant formats) include:immunohistochemical staining12.Note:For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. Nos. 430601 & 430606) are specially developed and recommended.The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10).
Host
Rat
Reactivity
Human
Application
ICFC - Quality tested
Platform ID
BAB490321528
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NamePE/Cyanine7 anti-human IL-10 Antibody anti-IL-10 - JES3-9D7
Cat. No.501420
HostRat
RRIDAB_2280292 (BioLegend Cat. No. 501419)AB_2125385 (BioLegend Cat. No. 501420)
IsotypeRat IgG1, κ
ReactivityHuman
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone NumberJES3-9D7
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetIL-10
ImmunogenCOS - expressed, recombinant human IL-10
PurityThe antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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