PE/Cyanine7 anti-mouse CD8b.2 Antibody anti-CD8b.2 - 53-5.8,BioLegend,140415

Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.

Host

Rat

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB813833754

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Contact

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Product Specifications
Scientific Background

Specifications

NamePE/Cyanine7 anti-mouse CD8b.2 Antibody anti-CD8b.2 - 53-5.8
Cat. No.140415
HostRat
RRIDAB_2564384 (BioLegend Cat. No. 140415)AB_2564385 (BioLegend Cat. No. 140416)
IsotypeRat IgG1, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone Number53-5.8
Concentration0.2 mg/ml
TargetCD8beta.2
ImmunogenMouse thymus or spleen
PurityThe antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.

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