PE/Cyanine7 anti-mouse I-Ab Antibody anti-I-Ab - AF6-120.1,BioLegend,116419
Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3(including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).Does not react with other haplotypes (e.g.,d, f, q, r, s).
Host
Mouse
Reactivity
Mouse
Application
FC - Quality tested
Platform ID
BAB016969995
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NamePE/Cyanine7 anti-mouse I-Ab Antibody anti-I-Ab - AF6-120.1
Cat. No.116419
HostMouse
RRIDAB_10575904 (BioLegend Cat. No. 116419)AB_10575296 (BioLegend Cat. No. 116420)
IsotypeMouse (BALB/c) IgG2a, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberAF6-120.1
Concentration0.2 mg/ml
TargetI-Ab
ImmunogenC57BL/10J splenocytes
PurityThe antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
The AF6-120.1 antibody reacts with the I-AbMHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2bbearing mice, and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2kand H-2uhaplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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