PE/Cyanine7 anti-mouse/rat/human CD27 Antibody anti-CD27 - LG.3A10,BioLegend,124216

Additional reported applications (for the relevant formats) include: functional assay1,3and immunohistomecial staining of acetone-fixed frozen sections2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 124243 & 124244).

Host

Armenian Hamster

Reactivity

Mouse, Rat, Human

Application

FC - Quality tested

Platform ID

BAB994769392

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NamePE/Cyanine7 anti-mouse/rat/human CD27 Antibody anti-CD27 - LG.3A10
Cat. No.124216
HostArmenian Hamster
RRIDAB_10645330 (BioLegend Cat. No. 124215)AB_10639726 (BioLegend Cat. No. 124216)
IsotypeArmenian Hamster IgG
ReactivityMouse, Rat, Human
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberLG.3A10
Concentration0.2 mg/ml
TargetCD27
ImmunogenArmenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA
PurityThe antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
FormulationPhosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, it is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells.

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