PE/Cyanine7 anti-rat CD45 Antibody anti-CD45 - OX-1,BioLegend,202213

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy (acetone fixed, ammonium-thiocyanate separated epidermal sheets)2, immunohistochemistry of acetone3-, isopentane-4, PLP5-, and n-hexane6fixed frozen sections and paraffin-embedded sections7,8, and partial inhibition of NK cell lysis of syngeneic tumor cell lines1.

Host

Mouse

Reactivity

Rat

Application

FC - Quality tested

Platform ID

BAB387048594

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Product Specifications
Scientific Background

Specifications

NamePE/Cyanine7 anti-rat CD45 Antibody anti-CD45 - OX-1
Cat. No.202213
HostMouse
RRIDAB_2174546 (BioLegend Cat. No. 202213)AB_2253306 (BioLegend Cat. No. 202214)
IsotypeMouse IgG1, κ
ReactivityRat
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberOX-1
Concentration0.2 mg/ml
TargetCD45
ImmunogenEnriched glycoprotein fraction from Wistar rat thymocytes
PurityThe antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD45 is a 180-220 kD protein also known as leukocyte common antigen (LCA). It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets; isoform expression depends on cell type, activation state, and cell maturation. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins including galectin-1, CD2, CD3, and CD4. The OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cellsin vitro.

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