PE/Dazzle 594 anti-human CD25 Antibody anti-CD25 - M-A251,BioLegend,356126

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections1and spatial biology (IBEX)2,3.The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.

Host

Mouse

Reactivity

Human

Application

FC - Quality tested

Platform ID

BAB495818888

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Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NamePE/Dazzle 594 anti-human CD25 Antibody anti-CD25 - M-A251
Cat. No.356126
HostMouse
RRIDAB_2563561 (BioLegend Cat. No. 356125)AB_2563562 (BioLegend Cat. No. 356126)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberM-A251
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD25
ImmunogenHuman PHA-induced lymphocyte cells
PurityThe antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.

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