PE/Dazzle 594 anti-mouse Ly-6A/E Sca-1 Antibody anti-Ly-6A/E - E13-161.7,BioLegend,122527

Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.The two Sca-1 recognizing clonesD7and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7

Host

Rat

Reactivity

Mouse

Application

FC -Quality tested

Platform ID

BAB117521707

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Product Specifications
Scientific Background

Specifications

NamePE/Dazzle 594 anti-mouse Ly-6A/E Sca-1 Antibody anti-Ly-6A/E - E13-161.7
Cat. No.122527
HostRat
RRIDAB_2687354 (BioLegend Cat. No. 122527)AB_2687355 (BioLegend Cat. No. 122528)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone NumberE13-161.7
Concentration0.2 mg/ml
TargetLy-6A/E
Immunogenmouse pre-T cells
PurityThe antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.

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