PE/Dazzle 594 anti-mouse Ly-6G Antibody anti-Ly-6G - 1A8,BioLegend,127647

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.Additional reported applications (for the relevant formats) include: immunohistochemistry9of frozen sections10and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended forin vivostudies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Host

Rat

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB231512023

BioLegend

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NamePE/Dazzle 594 anti-mouse Ly-6G Antibody anti-Ly-6G - 1A8
Cat. No.127647
HostRat
RRIDAB_2566318 (BioLegend Cat. No. 127647)AB_2566319 (BioLegend Cat. No. 127648)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone Number1A8
Concentration0.2 mg/ml
TargetLy-6G
ImmunogenLy-6G transfected EL-4J cell line.
PurityThe antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

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