PE/Fire™ 640 anti-mouse/human CD45R/B220 Antibody, CD45R/B220, RA3-6B2,BioLegend,103275

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroandin vivomodulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Host

Rat

Reactivity

Mouse, Human

Application

FC -Quality tested

Platform ID

BAB896194202

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NamePE/Fire™ 640 anti-mouse/human CD45R/B220 Antibody, CD45R/B220, RA3-6B2
Cat. No.103275
HostRat
RRIDAB_2860602 (BioLegend Cat. No. 103275)AB_2876407 (BioLegend Cat. No. 103276)
IsotypeRat IgG2a, κ
ReactivityMouse, Human
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone NumberRA3-6B2
Concentration0.2 mg/mL
TargetPTPRC
ImmunogenAbelson murine leukemia virus-induced pre-B tumor cells
PurityThe antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslprand MRL-Fasgldmice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

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