PE/Fire™ 744 anti-human CD86 Antibody, CD86, IT2.2,BioLegend,305466

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, Western blotting3, and blocking of T cell activation2,4,5. The Ultra-LEAF™purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).

Host

Mouse

Reactivity

Human, Cynomolgus, Rhesus

Application

FC - Quality tested

Platform ID

BAB802128111

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NamePE/Fire™ 744 anti-human CD86 Antibody, CD86, IT2.2
Cat. No.305466
HostMouse
RRIDAB_3720498 (BioLegend Cat. No. 305465)AB_3720498 (BioLegend Cat. No. 305466)
IsotypeMouse IgG2b, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberIT2.2
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD86
PurityThe antibody was purified by affinity chromatography and conjugated with PE/Fire™ 744 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.

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