PE anti-H2A.X Phospho Ser139 Antibody anti-Phosphorylated H2A.X - 2F3,BioLegend,613412

Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.