PE anti-Syk Phospho (Tyr296) Recombinant Antibody, Syk Phospho, QA20B02,BioLegend,949304

Syk is a non-receptor tyrosine kinase that is a member of the Syk family of tyrosine kinases, with broad expression in hematopoietic cells. Upon immune receptor engagement, phosphorylated ITAM motifs within the cytosolic region of the activated receptor become phosphorylated and recruit Syk to the activated receptor via SH2 domains. ITAM-bound Syk is catalytically active, transmitting signals to multiple downstream signaling partners that drives processes such as phagocytosis, proliferation, and differentiation.Recent evidence also suggests a role for Syk in non-immune cells. Elevated Syk activation is associated with B-cell malignancies, rheumatoid arthritis, and Alzheimer’s disease, and multiple Syk inhibitors are being developed in clinical trials. Syk activity and function are both regulated by tyrosine phosphorylation at multiple sites. Tyr323 and Tyr352 phosphorylation facilitate binding to downstream effectors, and phosphorylation of Tyr525/Tyr526 in the activation loop is required for kinase activity. Tyr296 phosphorylation has been reported in lung epithelial cancer cells, and has been shown to occur as part of an autophosphorylation mechanismin vitro. This site is embedded within a larger N-terminal linker region that is required for nuclear localization in breast cancer cells. Despite rapid autophosphorylation of this site upon Syk activation, it is not required for Syk activity.