PE anti-human CD172a/b SIRPalpha/beta Antibody anti-CD172a/b - SE5A5,BioLegend,323805

Clone SE5A5 recognizes a common epitope on SIRPa (90 kD) and SIRPß (50 kD)3. A high degree of homology has been found between SIRP family isoforms alpha and beta at the level of extracellular domains. Consequently, many anti SIRP antibody clones, such as SE5A5, have been reported to cross react with several SIRP isoforms1,4,5. It reacts with CD172a and has weak cross-reaction with CD172b. This antibody is able to block the binding of SIRPa (SIRPa1 and SIRPa2) to CD471,6.

Host

Mouse

Reactivity

Human

Application

FC - Quality tested

Platform ID

BAB464622605

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Product Specifications
Scientific Background

Specifications

NamePE anti-human CD172a/b SIRPalpha/beta Antibody anti-CD172a/b - SE5A5
Cat. No.323805
HostMouse
RRIDAB_830704 (BioLegend Cat. No. 323805)AB_830705 (BioLegend Cat. No. 323806)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberSE5A5
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD172a/b
ImmunogenNIH-3T3/hu-SIRPα cell line
PurityThe antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD172a, also known as signal-regulatory protein α (SIRPα), src homology 2 domain-containing phosphatase substrate-1 (SHPS1), PTPNS1, BIT, MFR, and P84, is a 75-110 kD transmembrane glycoprotein involved in receptor tyrosine kinase coupled signaling pathway. It belongs to the Ig superfamily and is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, and neurons. CD172a serves as a substrate of activated receptor tyrosine kinases (RTKs). The interaction of CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion, and phagocytosis. CD47 (IAP) is the extracellular ligand for CD172a. SIRPα was recently demonstrated to be a specifc marker for cardiomyocytes derived from human pluripotent stem cells2.

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