PE anti-human IL-3 Antibody anti-IL-3 - BVD3-1F9,BioLegend,500606
ELISA1-3,6or ELISPOT4Detection:The biotinylated BVD3-1F9 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified BVD8-3G11 (Cat. No. 500502) antibody as the capture antibody.Flow Cytometry:The fluorochrome-labelled BVD3-1F9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-3 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Additional reported applications (for the relevant formats) include: immunoprecipitation6, Western blotting6, neutralization1,6, immunohistochemical staining5,7of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Host
Rat
Reactivity
Human
Application
ICFC - Quality tested
Platform ID
BAB837522378
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NamePE anti-human IL-3 Antibody anti-IL-3 - BVD3-1F9
Cat. No.500606
HostRat
RRIDAB_2123712 (BioLegend Cat. No. 500606)
IsotypeRat IgG1, κ
ReactivityHuman
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone NumberBVD3-1F9
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetIL-3
ImmunogenYeast-expressed, recombinant human IL-3
PurityThe antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD3-1F9 antibody can neutralize the bioactivity of natural or recombinant IL-3.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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