PE anti-human/mouse CD3epsilon activation epitope Antibody anti-CD3e - APA1/1,BioLegend,362703
Clone APA1/1 recognizes an activation-dependent intracellular epitope of the CD3ε chain. Additional reported applications (for the relevant formats of this clone) include: immunofluorescent staining of paraformaldehyde fixed sections1,in vivotracking of T cells2, and Western blotting3. The LEAF purified format is recommended forin vivostudies.
Host
Mouse
Reactivity
Human, Mouse
Application
ICFC - Quality tested
Platform ID
BAB900980639
BioLegend
Contact
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Product Specifications
Scientific Background
Specifications
NamePE anti-human/mouse CD3epsilon activation epitope Antibody anti-CD3e - APA1/1
Cat. No.362703
HostMouse
RRIDAB_2564239 (BioLegend Cat. No. 362703)AB_2564240 (BioLegend Cat. No. 362704)
IsotypeMouse IgG1, κ
ReactivityHuman, Mouse
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone NumberAPA1/1
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD3epsilon
ImmunogenPurified denatured CD3 proteins from human thymus
PurityThe antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
The CD3ε chain of the CD3-TCR complex is a type I membrane protein that undergoes conformational change following ligation of the TCR-CD3 complex. This conformational change leads to exposure of the proline rich sequence in CD3ε and results in recruitment of Nck. It is expressed by all mature T cells and forms a heteromeric complex with CD3 α/β or γ/δ and ζ chains. It is important in T cell development, and deficiency of this gene has been associated with severe immunodeficiency.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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