PE anti-mouse CD1d Antibody anti-CD1d - K253,BioLegend,140805

Additional reported applications (for the relevant formats) include: ELISA1, immunofluorescence2to detect CD1d in the lipid rafts on the cell membrane, and block1CD1d recognition of aniNKT cell hybridoma.

Host

Mouse

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB616783326

BioLegend

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8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NamePE anti-mouse CD1d Antibody anti-CD1d - K253
Cat. No.140805
HostMouse
RRIDAB_10643277 (BioLegend Cat. No. 140805)
IsotypeMouse IgG1, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberK253
Concentration0.2 mg/ml
TargetCD1d
Immunogenα-GalCer:mCD1d complexes coupled to Mycobacterium tuberculosis PPD
PurityThe antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD1d is a type I transmembrane protein and member of the MHC family, with a molecular weight ranging from 43-49 kD, depending on the glycosylation degree. CD1d is expressed by antigen presenting cells such as dendritic cells, monocytes, macrophages and B cells; also expressed by thymocytes and intestinal epithelial cells. CD1d present glycolipids toiNKT cells that recognize them by their Vα14iTCR.

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