PE anti-mouse CD275 B7-H2, B7-RP1, ICOS Ligand Antibody anti-CD275 - HK5.3,BioLegend,107405
Additional reported applications (for the relevant formats) include:in vitroandin vivoblocking of ligand binding1,2. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107405) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107404) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 107408). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 107410) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Application
FC - Quality tested
Platform ID
BAB109936614

BioLegend
Contact
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Product Specifications
Scientific Background
Specifications
NamePE anti-mouse CD275 B7-H2, B7-RP1, ICOS Ligand Antibody anti-CD275 - HK5.3
Cat. No.107405
HostRat
RRIDAB_2248797 (BioLegend Cat. No. 107405)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberHK5.3
Concentration0.2 mg/ml
TargetCD275
ImmunogenMouse B7-RP1 transfected cell line
PurityThe antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
CD275 is a 40 kD protein, also known as ICOS ligand, B7-RP1, B7-H2, and B7h. It is a member of the immunoglobulin receptor superfamily expressed on splenic B cells, dendritic cells, and macrophages. The expression of this antigen is increased by TNF-α stimulation and is highly upregulated by local inflammation. B7RP1 binds to ICOS and co-stimulates T cell and B cell responses. Two isoforms of this protein have been reported to occur as a result of alternative splicing. These isoforms show differential tissue distribution.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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