PE anti-mouse CD284 TLR4 Antibody anti-CD284 - SA15-21,BioLegend,145404
Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.Additional reported applications (for the relevant formats) include: immunoprecipitation1and inhibition of LPS-induced hepatocyte apoptosis2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact ourcustom solutions team).
Host
Rat
Reactivity
Mouse
Application
FC - Quality tested
Platform ID
BAB089435195
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NamePE anti-mouse CD284 TLR4 Antibody anti-CD284 - SA15-21
Cat. No.145404
HostRat
RRIDAB_2561873 (BioLegend Cat. No. 145403)AB_2561874 (BioLegend Cat. No. 145404)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberSA15-21
Concentration0.2 mg/ml
TargetCD284
ImmunogenLPS-stimulated TLR4/MD-2/CD14 cotransfected NKR cells.
PurityThe antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells, and T cells. TLR4 has been recognized as critical for host recognition of LPS in conjunction with MD2.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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