PE anti-mouse IRF5 Antibody, IRF5, W16007B,BioLegend,158603

This clone was tested for ICC using RAW264.7 cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol. While both methods were compatible with IRF5 staining, the signal was weak and did not meet our sensitivity standards.This clone does not recognize human IRF5. For detection of human IRF5, please use BioLegend clone 11F4A09 (Cat. No. 652602).During product development testing of this clone for western blot, a ~37 kD protein of unknown origin was faintly detected. This protein may represent a minor IRF5 degradation product.

Host

Rat

Reactivity

Mouse

Application

ICFC -Quality tested

Platform ID

BAB828404228

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NamePE anti-mouse IRF5 Antibody, IRF5, W16007B
Cat. No.158603
HostRat
RRIDAB_2819919 (BioLegend Cat. No. 158603)AB_2876549 (BioLegend Cat. No. 158604)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationICFC -Quality tested
ClonalityMonoclonal
Clone NumberW16007B
Concentration0.2 mg/mL
TargetIRF5
ImmunogenMouse IRF5 recombinant protein (149-400 a.a.)
PurityThe antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

The interferon regulatory factor (IRF) family of transcription factors comprises nine members with conserved domains. IRF5 mediates the induction of proinflammatory cytokine, such as IL-6 and TNFa. IRF5 is highly expressed in monocytes, macrophages, B cells, and dendritic cells. Expression of IRF5 is associated with many autoimmune diseases.

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