PE anti-rat IFN-gamma Antibody anti-IFN-gamma - DB-1,BioLegend,507806

ELISA Capture1or ELISPOT Capture2:The purified DB-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated poly5109 antibody (Cat. No. 510901) as the detecting antibody and recombinant IFN-? (Cat. No. 565701) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry5:The fluorochrome-labeled DB-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-?-producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization3,4:The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of rat IFN-? bioactivityin vivoandin vitro(Cat. No. 507808).Additional reported applications (for the relevant formats) include:Western blotting1, and immunohistochemistry2of paraformaldehyde-fixed, saponin-treated frozen tissue sections.