Pacific Blue anti-human CD45 Antibody anti-CD45 - HI30,BioLegend,304021

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Host

Mouse

Reactivity

Human

Application

FC - Quality tested

Platform ID

BAB856413306

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Headquarters

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NamePacific Blue anti-human CD45 Antibody anti-CD45 - HI30
Cat. No.304021
HostMouse
RRIDAB_493654 (BioLegend Cat. No. 304021)AB_2174123 (BioLegend Cat. No. 304029)AB_493655 (BioLegend Cat. No. 304022)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberHI30
Concentrationµg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD45
PurityThe antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Formulationtest size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

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