Pacific Blue™ anti-mouse GM-CSF Antibody, GM-CSF, MP1-22E9,BioLegend,505425

ELISA or ELISPOT Capture1,3-5:The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry8:The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations.Neutralization2-4:The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended forin vivoandin vitroneutralization (Cat. No. 505408).Additional reported applications (for the relevant formats) include:immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,6,7, and immunocytochemistry8.