Pacific Blue anti-mouse H-2Kb Antibody anti-H-2Kb - AF6-88.5,BioLegend,116514

Additional reported applications (for the relevant formats) include: immunoprecipitation2and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.Does not cross-react with other haplotypes (e.g.,d, f, j, k, p, q, r, s, u, v).

Host

Mouse

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB267094935

BioLegend

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NamePacific Blue anti-mouse H-2Kb Antibody anti-H-2Kb - AF6-88.5
Cat. No.116514
HostMouse
RRIDAB_1967103 (BioLegend Cat. No. 116513)AB_1967129 (BioLegend Cat. No. 116514)
IsotypeMouse (BALB/c) IgG2a, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberAF6-88.5
Concentration0.5 mg/ml
TargetH-2Kb
ImmunogenC57BL mouse splenocytes
PurityThe antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

The AF6-88.5 antibody reacts with H-2KbMHC class I alloantigen expressed on nucleated cells from mice of the H-2Kbhaplotype. H-2Kbis involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

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