PathScan®Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) Sandwich ELISA Kit#12001,Cell Signaling Technology (CST),12001
PathScan®Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) Sandwich ELISA Kit recognizes endogenous levels of phospho-SMAD2 (Ser465/467) and SMAD3 (Ser423/425) proteins in human cells, as shown in Figure 1. This kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Reactivity
Human, Mouse, Mink
Platform ID
BAB709416312
Cell Signaling Technology (CST)
Contact
Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355
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Specifications
Scientific Background
Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8).Heldin, C.H. et al. (1997)Nature390, 465-71.Attisano, L. and Wrana, J.L. (1998)Curr Opin Cell Biol10, 188-94.Derynck, R. et al. (1998)Cell95, 737-40.Massagué, J. (1998)Annu Rev Biochem67, 753-91.Whitman, M. (1998)Genes Dev12, 2445-62.Wrana, J.L. (2000)Sci STKE2000, re1.Attisano, L. and Wrana, J.L. (2002)Science296, 1646-7.Moustakas, A. et al. (2001)J Cell Sci114, 4359-69.Alternate NamesDKFZp586N0721; DKFZp686J10186; hMAD-2; hMAD-3; hSMAD2; hSMAD3; HSPC193; HsT17436; JV15-2; JV18; JV18-1; LDS1C; LDS3; MAD homolog 2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; Mad protein homolog; Mad-related protein 2; MAD, mothers against decapentaplegic homolog 2; MAD, mothers against decapentaplegic homolog 3; Mad3; MADH2; MADH3; MADR2; MGC22139; MGC34440; MGC60396; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 2; Mothers against DPP homolog 3; Sma- and Mad-related protein 2; SMA- and MAD-related protein 3; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3; SMAD, mothers against DPP homolog 2; SMAD, mothers against DPP homolog 3; SMAD2; SMAD3
Synonyms
DKFZp586N0721; DKFZp686J10186; hMAD-2; hMAD-3; hSMAD2; hSMAD3; HSPC193; HsT17436; JV15-2; JV18; JV18-1; LDS1C; LDS3; MAD homolog 2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; Mad protein homolog; Mad-related protein 2; MAD, mothers against decapentaplegic homolog 2; MAD, mothers against decapentaplegic homolog 3; Mad3; MADH2; MADH3; MADR2; MGC22139; MGC34440; MGC60396; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 2; Mothers against DPP homolog 3; Sma- and Mad-related protein 2; SMA- and MAD-related protein 3; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3; SMAD, mothers against DPP homolog 2; SMAD, mothers against DPP homolog 3; SMAD2; SMAD3
Category Paths
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